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ISSN : 1225-0171(Print)
ISSN : 2287-545X(Online)
ISSN : 2287-545X(Online)
Korean Journal of Applied Entomology Vol.63 No.4 pp.367-371
DOI : https://doi.org/10.5656/KSAE.2024.11.0.052
DOI : https://doi.org/10.5656/KSAE.2024.11.0.052
An Improved Mass Rearing Method for Western Flower Thrips, Frankliniella occidentalis
Abstract
Western flower thrips (Frankliniella occidentalis), commonly known as a sap-sucking insect pests that causes both direct and indirect damage to a variety of crops. It is widely reared for studies on insecticide resistance and other experimental purposes. In this study, we developed an improved rearing method that saves both time and space. The notable improvements include using harvested cotyledons without washing, maintaining proper moisture levels in the rearing containers to prevent drying of the cotyledons during oviposition, reducing moisture from the egg stage to the late nymphal stage to avoid excessive humidity, and slightly increasing moisture levels from the late nymphal stage to pupation. This method allows for the layering of rearing containers during the nymph and pupal stages, and egg-laying containers can also be arranged in a stepwise manner during the adult stage to maximize space efficiency. By adopting this improved approach, we significantly reduced preparation time for rearing and egg-laying containers and decreased the required rearing area by up to 93%. The number of adults produced per container ranged from 50 to 100, with some containers yielding as many as 150 to 200 adults.
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Vol. 40 No. 4 (2022.12)
Frequency Quarterly
Doi Prefix 10.5656/KSAE
Year of Launching 1962
Publisher Korean Society of Applied Entomology
Online Submission
submission.entomology2.or.kr
KSAE
The Korean Society of Applied Entomology
