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ISSN : 1225-0171(Print)
ISSN : 2287-545X(Online)
Korean Journal of Applied Entomology Vol.63 No.1 pp.67-72

Two New Records of Soil-inhabiting Mesostigmatic mites (Acari: Mesostigmata) in Korea

Jaeseok Oh1, Seunghwan Lee1,2*
1Laboratory of Insect Biosystematics, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea
2Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
August 10, 2023 January 4, 2024 February 21, 2024


In this study, two mesostigmatic mites, Holostaspella crenulata Krantz, 1967 (Machrochelidae) and Lasioseius floridensis Berlese, 1916 (Blattisociidae) are recognized in Korea for the first time. The diagnoses, distributional data, drawing plates, and microscopic photographs are provided for each species.

한국의 중기문응애류(응애아강: 중기문응애목) 미기록 2종 보고

오재석1, 이승환1,2*
1서울대학교 농생명공학부 곤충계통분류학 실험실
2서울대학교 농업생명과학연구원


본 연구를 통해 국내에 분포하는 중기문응애류 중 파리응애과의 Holostaspella crenulata Krantz, 1967 (톱니무늬파리응애, 신칭) 와 화살응애 과의 Lasioseius floridensis Berlese, 1916 (가슴선화살응애, 신칭) 를 처음으로 확인하고, 각 종에 대한 분류학적 진단과 분포정보, 성충의 현미경사 진과 도판을 제시하였다.

    The order Mesostigmata includes 180 families, 869 genera, 11,408 species worldwide (Beaulieu et al., 2011), with 307 species of 111 genera and 33 families recorded in Korea (NIBR, 2021). The genus Holostaspella, which belongs to the family Macrochelidae, has over 30 species worldwide (Hartini and Takaku, 2010), but only two species, H. ornata (Berlese, 1904); H. scatophilaTakaku, 1994 have been recorded in Korea (Lim and Lee, 2005;Hartini and Takaku, 2010;Keum et al., 2016). Most of Macrochelid mites are associated with carrion insects (Krantz, 1962a;Takaku, 1994;Hartini and Takaku, 2003;Arriaga-Jimenez et al., 2014;Knee, 2017), however, some species of Holostaspella has been observed from leaflitters, pasture, and fungus (Krantz, 1967;Halliday, 1988, 2000;Plumari, 2010;Takaku et al., 2012;Özbek, 2017).

    The genus Lasioseius, which belongs to the family Blattisociidae, includes 206 species worldwide, with five species, (L. furcisetus Athias-Henriot, 1959; L. porulosus De Leon, 1963; L. sugawarai Ehara, 1964; L. tomokoae Ishikawa, 1969; L. yousefi Athias-Hentiot, 1959) reported in Korea (Lee and Lee, 1998;Kontschán et al., 2015;Faraji, 2021). Among those Korean species, L. lasiodactyli Ishikawa, 1969 was synonymized with L. youcefi Athias-Henriot, 1959 by Christian and Karg (2006). Although this genus is typically free-living, they have also been found in grasslands, upper soil, crop fields, and even animal nests (Christian and Karg, 2006). In this study, we report two new record species, H. crenulataKrantz, 1967, and L. floridensisBerlese, 1916, along with diagnoses and illustrations. Additionally, key to the Korean species of each genus is also provided.

    Material and Methods

    The samples were separated from the leaf-litters and insectrearing cage by using Berlese-Tullgren funnel trap (60 W, 48 h). Mites were cleared in lactic acid with 70°C of dry bath and mounted in PVC medium (Downs, 1943). The identifications and capturing digital images of the specimens were performed with Olympus BX53 DIC (differential interference contrast) microscope with Olympus DP27 camera and Cellsens standard 3.1 software. Line drawing plates were prepared with Adobe Illustrator CC 22.0.1 program based on digital images. The length of all shields was measured from the anterior-median peaks to the posterior-median peaks, and the width was taken from the widest margin of the shields, respectively. The length of the legs was measured from coxa to the apex of the tarsus, excluding the pre-tarsus. The nomenclature used for the dorsal chaetotaxy follows Lindquist and Evans (1965), the other morphological terms follow Evans (1963), Evans and Till (1979), and Krantz and Walter (2009).

    The specimens examined are deposited at the National Institute of Biological Resources (NIBR) and the Insect Biosystematics Lab., Seoul National University (SNU), Seoul, Republic of Korea.

    Systematic Accounts

    Family Macrochelidae Vitzthum, 1930

    Genus HolostaspellaBerlese, 1903

    HolostaspellaBerlese, 1903: 241.

    PrholaspinaBerlese, 1918: 175.

    AreolaspisTrägårdh, 1952: 61.

    Holostaspella crenulataKrantz, 1967 톱니무늬파리응애(신 칭) (Figs. 1-3)

    Holostaspella crenulataKrantz, 1967: 151.

    Diagnosis. Female. Body orange-colored, circular shape, 527-641 μm in length, 495-504 μm in width. Dorsal shield without anterior projection, bearing 28 pairs of slightly pectinated setae (41-63 μm) (Fig. 1A). Sternal setae smooth acicular-liked, moderate length. Ventrianal shield oval-shaped with four pairs of acicular setae, nine to ten bipectinated setae on ventral integument (Fig. 1B). Thumb and hook-liked projections on each segment of leg II, most leg setae pectinated (Fig. 3D). In gnathosoma, four pairs of simple gnathosomal setae and five rows of deutosternal grooves with numerous denticles (Fig. 3B). Tripartited epistome with folked medial projection (Fig. 3A). Fixed digit of chelicera with simple pillus dentilis, movable digit of chelicera bidentate, dagger-shaped cheliceral seta, tassel-liked arthropodial brush present (Fig. 1C). Surface of all shields covered with punctured thick ridges (Figs. 2A, B).

    Male. Body size 438 μm in length, 373 μm in width. In ventral, holoventral shield covered whole ventral portion, surface of shield covered with same reticulations as in female (Figs. 2C, D). Genital orifice located on anterior margin of shield, seven pairs of simple setae bearing on shield excluding para and post anal setae (Fig. 1D). Spermatodactyl curved backward (sickle-shaped) (73-77 μm) (Fig. 3C). In legs, most setae acicular-shaped except for some dorsum setae and femur-tibia II with projections. Other morphological features the same as in females.

    Specimens examined. 12♀♀, 1♂, Hangye-ri 854, Bukmyeon, Inje-gun, Gangwon-do, Korea, 24. viii. 2020, from humid leaf litter, leg. Jaeseok Oh.

    Distributions. Yap island (Micronesia) (Krantz, 1967); Australia (Halliday, 1988); Philippines (Takaku et al., 2012); Korea.

    Remarks.Halliday (1988) described that all of the male’s legs setae are smooth and pointed. However, we observed some slightly modified setae in our male specimen. femur I: pd1, pd2; trochanter II: ad; femur II: ad1, ad2, pd1, pd2, and pv1 (blunted); genu II: ad1, ad2, pd1, and pd2; tibia II: ad1, ad2, pd1, and pd2; genu III: ad1, ad2, pd1, and pd2; tibia III: ad, pd1, and pd2; tarsus III: ad3, ad4, pd3, and pd4; trochanter IV: ad; femur IV: al, ad1, and pd; genu IV: al, ad1, ad2, pd1, and pd2; tibia IV: al, ad1, ad2, pd, and pl; tarsus IV: ad3, ad4, pd3, and pd4.

    Key to species of the genus Holostaspella in Korea based on the female

    1. Anterior margin of dorsal shield with apical projection and setae j1 ·································································· 2

      • - Anterior margin of dorsal shield without apical projection ···································································· H. crenulata

    2. Dorsal setae bipectinated except simple setae on medial region; sternal setae st1-st3 short and smooth; ventrianal shield rectangular-shaped, longer than wide ·················· ································································· H. scatophila

      • - Dorsal setae slightly pectinated; sternal setae st1 plumose, st2 and st3 simple; ventrianal shield expanded oval-shaped ········································································· H. ornata

    Family Blattisociidae Garman, 1948

    Genus LasioseiusBerlese, 1916

    LasioseiusBerlese 1916: 33.

    AceoseiusSellnick, 1941: 149.

    BorinquolaelapsFox, 1946: 450.

    HyattellaKrantz, 1962b: 6.

    GnorimusChaudhri, 1975: 100

    IndiraseiusDaneshvar, 1987: 32.

    NeolaspinaHalliday, 1995: 213.

    Lasioseius floridensisBerlese, 1916 가슴선화살응애(신칭) (Figs. 4-5)

    Lasioseius (Zercoseius) penicilliger var. floridensisBerlese 1916: 44.

    Lasioseius arboreusChant, 1963: 291.

    Lasioseius fimetorumKarg, 1971: 248.

    Diagnosis. Female. Body pale-yellow colored, rectangular shape, 407 μm in length, 220 μm in width. Dorsal shield not covered whole dorsum, surface of the shield covered with scalelike reticulation. Dorsal shield bearing 36 pairs of tricarinate and pointed-pectinated setae except acicular-shaped z1, s2, r2, and J5. Marginal setae r2-r4 bearing on dorsal shield, r5, r6, and R1-R6 situated in the lateral cuticle (Figs. 4A, 5A). Surface of sternal and genital shield covered with dotted patterns, anteromedial part of sternal shield with irregular crack. Ventrianal shield with four pairs of simple setae, surface of shield covered with distinctive lineate reticulations (Figs. 4B, 5B). In gnathosoma, seven rows of deutosternal grooves with numerous denticles (Fig. 5C). Branched epistome with a pair of serrated lateral branches and slightly forked median projection (Fig. 5D).

    Specimens examined. 1♀, Songcheon-dong, Andong-si, Gyeongsangbuk-do, Korea, 03. vii. 2019, from dried sawdust bedding in Dorcus rectus rearing cage, leg. Jaeseok Oh & Sehyeon Bang.

    Distribution. Brazil, Canada, Germany, Mexico, and the U.S.A. (Britto et al., 2011); Korea.

    Remarks.Christian and Karg (2006) listed L. floridensis as one of the species under inquiry within the genus Lasioseius. Subsequently, Britto et al. (2011) reestablished L. floridensis as a valid species, providing a detailed description and several plates illustrating both sexes.

    Key to species of the genus Lasioseius in Korea based on the female

    1. Dorsal shield with more than 30 pairs of setae ············· 2

      • - Dorsal shield with less than 30 pairs of setae; ventrianal shield with six pairs of setae ···························· L. youcefi

    2. Dorsal shield with 31 pairs of setae (16 pairs on podonotal region) ························································ L. porulosus

      • - Dorsal shield with 35 or 36 pairs of setae (20-21 pairs on podonotal region) ·························································· 3

    3. Dorsal shield with 35 pairs of setae (20 pairs on podonotal) ·························································· L. tomokoae

      • - Dorsal shield with 36 pairs of setae (21 pairs on podonotal) ······················································································ 4

    4. Ventrianal shield with six pairs of setae; sternal shield with dotted reticulation ·················································· ··································································· L. furcisetus

      • - Ventrianal shield with four pairs of setae; sternal shield without dotted reticulation ············································ 5

    5. Ventral integument with five pairs of setae; sternal shield without irregular crack ······························ L. sugawarai

      • - Ventral integument with six pairs of setae; sternal shield with irregular crack on Anteromedial part ·· L. floridensis


    We deeply thank Mr. Sehyeon Bang for collecting samples. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF2020R1I1A2069484), and also supported by the research grant from the National Institute of Biological Resources (NIBR) of the Ministry of Environment (MOE) in Korea (NIBR202002204, NIBRE202404).

    Statements for Authorship Position & Contribution

    • Oh, J.: Seoul National University, Student in Ph.D.; Wrote the manuscript, collecting samples, identification, drawing plates

    • Lee, S.: Seoul National University, Professor; Designed the research, critically revised the manuscript

    All authors read and approved the manuscript.



    line drawing plates of Holostaspella crenulata Krantz. A, female dorsum; B, female venter; C, female chelicera; D, male venter. Scale bar for A, B, and D = 100 μm; C = 50 μm.


    micro-photographs of Holostaspella crenulata Krantz. A, female dorsum; B, female venter; C, male dorsum; D, male venter. Scale bar for A-D = 100 μm.


    micro-photographs with a line drawing of Holostaspella crenulata Krantz. A, female epistome; B, female ventral gnathosoma; C, male spermatodactyl; D, female projections on leg II. Scale bar for A and B = 50 μm ; C and D = 100 μm


    line drawing of Lasioseius floridensis Berlese, female. A, dorsal view; B, ventral view. Scale bar for A and B = 100 μm


    micro-photographs with a line drawing of Lasioseius floridensis Berlese, female. A, dorsal view; B, ventral view; C, ventral gnathosoma; D, epistome. Scale bar for A and B = 100 μm; C and D = 50 μm



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    Vol. 40 No. 4 (2022.12)

    Journal Abbreviation Korean J. Appl. Entomol.
    Frequency Quarterly
    Doi Prefix 10.5656/KSAE
    Year of Launching 1962
    Publisher Korean Society of Applied Entomology
    Indexed/Tracked/Covered By