Journal Search Engine
Search Advanced Search Adode Reader(link)
Download PDF Export Citaion korean bibliography PMC previewer
ISSN : 1225-0171(Print)
ISSN : 2287-545X(Online)
Korean Journal of Applied Entomology Vol.57 No.2 pp.117-121
DOI : https://doi.org/10.5656/KSAE.2018.02.1.052

First Report of Genus Canephora (Lepidoptera: Psychidae) in Korea, with a Newly Recorded Species, C. pungelerii

Seung Jin Roh, Bong-Kyu Byun1*
Division of Forest Biodiversity, Korea National Arboretum, Pocheon 11186, Korea
1Department of Biological Science and Biotechnology, Hannam University, Yuseong-Gu, Daejeon, 34054, Korea
Corresponding author:bkbyun@hnu.ac.kr
November 30, 2017 February 1, 2018 March 10, 2018

Abstract


The genus Canephora Hübner is reported for the first time from Korea with a newly recorded species, Canephora pungelerii (Helaerts). All available information, including the collection localities and illustration of female, are presented. DNA barcode for precise identification of the species is also provided.



한국미기록 작은검정주머니나방속(나비목: 주머니나방과) 작은날개검정주머니나방 보고

노 승진, 변 봉규1*
국립수목원 산림생물조사과
1한남대학교 생명시스템과학과

초록


금번 연구를 통해 작은검정주머니나방속이 국내에서 처음 발견되어 보고한다. 작은검정주머니나방속 내 작은날개검정주머니나방에 대한 암 컷 형태 특징, 채집지 정보 등을 포함한 이용가능한 모든 정보를 제공하였다. 또한 신속한 종 동정을 위한 DNA 바코드 정보를 작성하였다.



    The family Psychidae is a medium-sized family consisting of 241 named genera and 1,350 species (Sobczyk, 2011; van Nieukerken et al., 2011). Most psychids make various cases or bags at different larval stages (Sugimoto, 2009a, 2009b). Also, males have wings, whereas most females cannot fly because of their wings have been reduced (Niitsu and Kobayashi, 2008; Roh et al., 2016).

    In Korea, Roh et al. (2016) reviewed nine species including a new species, Psyche yeongwolensisByun and Roh, 2016 and a newly recorded species, Proutia maculatellaSaigusa and Sugimoto, 2014. Later, Roh and Byun (2016) recorded Ceratosticha leptodetaMeyrick, 1935 new for Korea. Recently, five more species were reported: Bacotia sakabeiSeino, 1981 (Roh and Byun, 2017a), Bruandella niphonica (Hori, 1926) and Proutia nigraSaigusa and Sugimoto, 2014 (Roh and Byun, 2017b), Dahlica (Dahlica) somae Roh and Byun, 2018 and Dahlica (Dahlica) ochrostigma Roh and Byun, 2018 (Roh et al., 2018). Consequently, 15 species in total are now known from Korea.

    The genus Canephora, belonging to the subfamily Oiketicinae, was established by Hübner (1822) based on the type species Phalaena (Bombyx) unicolorHufnagel, 1766. Heylaerts (1900) described a new species, Chalia pungelerii from Japan. Later, Chalia pungelerii changed into Canephora pungelerii by Inoue (1981). Poda (1761) described a new species of Canephora, C. hirsuta from Austria. Thus, a total of two species of Canephora have been recorded worldwide to date (Sobczyk, 2011).

    In this study, we report a newly recorded species of the genus Canephora, C. pungelerii from Korea. All available information is presented, including the collection locations, and illustration of female. DNA barcode is also provided for precise identification of each species.

    Materials and Methods

    The material examined in this study is preserved in the Systematic Entomology Laboratory, Hannam University (SEL/ HNU), Daejeon, Korea. Females were dissected and examined after mounting with euparal solution. Photographs were taken using a Pax cam digital camera (PAXcam Microscope Cameras Co., Chicago, IL, USA) attached to a Carl Zeiss Axio Imager A1 microscope (Carl Zeiss Ltd., Cambridge, MA, USA). Terminology and morphological characters of the females follows Sugimoto and Saigusa (2001).

    Genomic DNA was extracted from the thorax part of an immersion specimen, preserved in 100% alcohol using a Genomic Cell/Tissue Spin Mini Kit (Mbiotech, Inc., Hanam, Korea), according to the manufacturer’s protocol. A specimen was sequenced for, the 658 bp fragment of the mitochondrial cytochrome c oxidase I (COI) gene, the DNA barcode, was amplified using the primer pair Lep F1 and Lep R1 (Hebert et al., 2004). PCR conditions for amplification followed the manufacturer’s protocol (Platinum Taq, Invitrogen, Carlsbad City, CA, USA). Amplicons were purified using the QIAquick® PCR purification kit (QIAGEN, Inc.) and directly sequenced at Genotech Corp. (Yuseong-gu, Daejeon, Korea). Contigs were assembled using CodonCode aligner version 2.0.6 (CodonCode Co., Centerville City, MA, USA). Successful sequences were submitted to GenBank.

    Taxonomic accounts

    Order Lepidoptera Linnaeus, 1758.

    Family Psychidae Boisduval, 1829: 44.

    Subfamily Oieketicinae Herrich-Schäffer, 1855: 48.

    Genus Caenephora Hübner, 1822. 작은검정주머니나방속(신칭) Type species: Phalaena (Bombyx) unicolorHufnagel, 1766>

    Canephorapugelerii (Butler, 1881) 작은날개검정주머니나방 (신칭)

    Chalia pungelerii Heyaerts, 1900: 190. Type locality: Japan.

    Eumeta pugeleri: Tutt, 1900: 434.

    Eurycytarus andrewsi Wileman, 1911: 347.

    Canephora pungeleri: Dierl, 1970: 170.

    Canephora pungelerii: Inoue, 1981; 197-198.

    Adult. Female (Fig. 1). Length 8.4-9.1 mm, vermiform, cylindrical, nearly same thickness from the metathorax to the 7th abdominal segment, 9 + 10th abdominal segments with short papillae anales. Coloration: Head with reddish brown and shiny vertex. Meso and metanotum red-brown. Membranous areas of abdomen yellowish brown. 7th abdominal segment pale yellow. Structure: Head (Fig. 1B) reduced in size, about 0.07 times as long as body length; apical part of vortex with few short hairs, mouth part slightly degenerated, antennae very short; well-developed with 2 segments, eye-spots blackish. Thorax (Fig. 1C and D) small, 0.16 times as long as body length, dorsal margin weakly curved, prothorax narrower than meso and metathorax, lateral part of metathorax with degenerated wings and few small setae, forewing narrow than hindwing. Legs almost degenerated with very short, present 2 segments (consist of 2 short segments, basal part with one femur and apical part with one tibia (Sugimoto and Saigusa, 2001)). Abdomen well developed, cylindrical and 5.14 times as long as body length, first abdominal segment well sclerotized, second to 6th abdominal segments almost membranous and second segment shorter than 3 to 6th segments, seventh abdominal segment nearly 1.4 times as long as the 6th segment and wide, dorsal part of 7th segment (corethrogyne or anal hair tuft) densely covered reddish brown hairs (but macerated condition), eighth abdominal segment very short, apophysis anterior parts invaginated from anterior margin of sclerite and very short, ostium bursa slightly thickness, 9th and 10th abdominal segments present papillae anales (ovipositor), papillae anales sclerotized and slightly short.

    Male. Unknown.

    Larval case (Fig. 2). It makes a relatively wide cylindrical case, which is slightly tapered to the lower part and covered with pieces of leafs arranged longitudinally.

    Host plants (Fig. 2). It mainly feeds on Gramineae (Seino, 1975). In this study, Rhododendron indicum (L.) Sweet (Ericaceae) is reported for the first time from Korea as a host plant of C. pungelerii.

    Material examined. 2♀, Jangneung, Yeongwol-gun, GW, 20. vi. 2014 (B.K. Byun and S.J. Roh), slide no. KNAEFSJ01; 2♀, Jeonmin-dong, Yuseong-gu, Daejeon, 20. v. 2015 (S.J. Roh), slide no. KNAEFSJ02; 1♀, Jeonmin-dong, Yuseong-gu, Daejeon, 1. vi. 2015 (S.J. Roh); 1♀, Jeonmin-dong, Yuseong-gu, Daejeon, 2. vi. 2015 (S.J. Roh), slide no. KNAEFSJ03-coll. SEL/HNU.

    Distribution. Korea (new record), Japan.

    DNA barcode. DNA barcode sequence was generated (accession no. MG563819). Multiple alignments using the BLAST tool in the NCBI database showed the following species as nearest neighbor: Canephorapugelerii (locality of reference data from Japan) 98%.

    Acknowledgements

    We thank to Ms. Kim DS, Choi HR, and Mr. Kim SY, Systematic Entomology Lab., Hannam University, for help in larval rearing. This study was carried out with the support of the ‘R&D Program for Forest Science Technology (Project No. 2017042A00-1823-CA01)’ provided by Korea Forest Service (Korea Forestry Promotion Institute) and Korea National Arboretum (Project No. KNA1-1-20, 16-1).

    KSAE-57-117_F1.gif

    Female of Canephora pungelerii : A, Ventral aspect; B, Head; C, Thorax; D, Lateral aspect; E, Thorax, dorsal aspect; F, Wing; G, Genitalia (abbreviation, A (abdomen); T1 (prothorax); T2 (mesothorax); T3 (metathorax)).

    KSAE-57-117_F2.gif

    Larval case of Canephora pungelerii: A, Feeding on Rhododendron indicum (L.); B, Pupation (fixed the larval case on the fence).

    Reference

    1. J.B.A.D. Boisduval (1840) Genera et Index methodicus Europaeorum Lepidopterorum., Roret, ; pp.1-238(Original work published 1829)
    2. A.G. Butler (1881) Description of new genera and species of Heterocerous Lepidoptera from Japan., Trans. R. Entomol. Soc. Lond., Vol.1881 ; pp.21-23
    3. W. Dierl (1970) Flugzeit und Augengröße als systematisches Merkmal der Psychidae., Mitteilungen der Münchner Entomologischen Gesellschaft, Vol.59 ; pp.168-173
    4. G.A.W. Herrich-schäffer (1855) Systematische Bearbeitung der Schmetterlinge von Europa zugleich als Text, Revision und Supplement zujakob Hbners Sammlung europischer Schmetterlinge. Sechster und letzter Band G.J. Manz, Nachtrag zumersten Band, Regnsburg, pp. 81-152.,
    5. F.J.M. Heylaerts (1900) Remarques psychidilogiques et descriptions de nouvelles espèces et variétés., Annales de la Société Entomologique de Belgique, Vol.34 ; pp.clxxx-clxxxiii
    6. H. Hori (1926) A new Psychid from Japan., KontyA", Vol.1 ; pp.28-30
    7. J. HA1/4bner (1822) Systematisch-Alphabetisches verzeichniss aller bisher bei den Fürbildungen zur Sammlung europäischer Schmetterlinge angegebenen Gattungsbennungen., ; pp.1-81
    8. T. Hufnagel (1766) Tabelle von den Tagevogeln derhiesigen Gegend, worauf denen Liebhabern der Insekten Beschaffenheit, Zeit, Ort und andere Umstande der Raupen und der daraus entstehenden Schmetterlinge bestimmt werden. Berlinisches Magazin, oder gesammlete [sic] Schriften und Nachrichten fur die Liebhaber der Arzneywissenschaft, Naturgeschichte und der angenehmen Wissenschaften uberhaupt 2(1), 54-90.,
    9. H. Inoue (1981) Synonymy of Chalia pungelerii [sic!] Heyl., 1900 and Eurycyttarus andrewsi Wileman, 1911., Jpn. Heterocerists J., Vol.113 ; pp.197-212
    10. C. Linnaeus (1816) 1758. Systema Naturae per regna tria naturae, secundumclasses, ordines, genera,species, cum characteribus, differentiis, synonymis, locis. Editio Decima, Reformata. Holmiaep S.Ochsenheimer, F., Die Schmetterlige von Europa,
    11. E. Meyrick (1935) Exotic Microlepidoptera., Taylor and Francis, Vol.Vol. 4 ; pp.577-608
    12. E.J. van Nieukerken , L. Kaila , Z.Q. Zhang (2011) Animal biodiversity: an outline of higher-level classification and survey of taxonomic richness. Zootaxa 3148,, ; pp.212-221
    13. S. Niitsu , Y. Kobayashi (2008) The developmental process during metamorphosis that results in wing reduction in females of three species of wingless-legged bagworm moths, Taleporia trichopterella, Bacotia sakabei and Proutia sp. (Lepidoptera: Psychidae)., Eur. J. Entomol., Vol.105 ; pp.697-706
    14. N. Poda von Neuhaus (1761) Insecta Musei Graecensis, quae in ordines, genera et species juxta Systema Naturae Linaei digessit., Widmanstad. Graecii, ; pp.1-127
    15. S.J. Roh , G. Banasiak , B.K. Byun (2016) A new and an unrecorded species of the family Psychidae (Lepidoptera) from Korea, with an annotated catalogue., J. Nat. Hist., Vol.50 (11-12) ; pp.669-680
    16. S.J. Roh , B.K. Byun (2016) Discovery of Ceratosticha leptodeta Meyrick (Lepidoptera: Psychidae) from Korea., J. Asia-Pac. Biodivers., Vol.9 (1) ; pp.91-93
    17. S.J. Roh , B.K. Byun (2017) First discovery of the Lichen-Feeding Moth Bacotia sakabei (Lepidoptera: Psychidae) from Korea. Anim. Syst., Evol. Divers., Vol.33 (1) ; pp.60-64a
    18. S.J. Roh , B.K. Byun (2017) Two species of the subfamily Psychinae (Lepidoptera: Psychidae) new to Korea., J. Asia-Pac. Biodivers., Vol.10 (2) ; pp.224-227b
    19. S.J. Roh , B.W. Lee , B.K. Byun (2018) Two new species of the genus Dahlica Endelein (Lepidoptera, Psychidae) from Korea., ZooKeys, Vol.733 ; pp.49-64
    20. T. Saigusa , M. Sugimoto (2014) Japanese species of the genus Proutia Tutt, 1899 (Lepidoptera: Psychidae)., Zootaxa, Vol.3869 ; pp.143-152
    21. A. Seino (1975) A new psychid species of Bacotia from Japan (Lepidoptera). Ty to Ga 31, 121-125.,
    22. A. Seino (1981) A new psychid species of Bacotia from Japan (Lepidoptera)., TyA' to Ga, Vol.31 ; pp.121-125
    23. T. Sobczyk (2011) World catalogue of insects; Vol.10 Psychidae (Lepidoptera). Apollo Books, stenstrup, pp. 1-467.,
    24. M. Sugimoto (2009) A comparative study of larval cases of Japanese Psychidae (Lepidoptera)., Jpn. J. Entomol. (NS), Vol.12 ; pp.1-15a
    25. M. Sugimoto (2009) A comparative study of larval cases of Japanese Psychidae (Lepidoptera)., Jpn. J. Entomol. (NS), Vol.12 ; pp.17-29b
    26. M. Sugimoto , T. Saigusa (2001) External morphology and musculature of the female of a specialized psychid, Acanthopsyche nigraplaga (Wileman) (Lepidoptera, Psychidae). Trans. Lipid., Soc. Jpn., Vol.52 (4) ; pp.313-331
    27. J.W. Tutt (1900) A natural history of the British Lepidoptera 2., Swan Sonnenschein & Co., ; pp.1-584
    28. A.E. Wileman (1911) New and unrecorded species of Lepidoptera Heterocera from Japan., Trans. R. Entomol. Soc. Lond., Vol.59 ; pp.189-407