Journal 
The family Psychidae is a medium-sized family consisting of 241 named genera and 1,350 species (Sobczyk, 2011; van Nieukerken et al., 2011). Most psychids make various cases or bags at different larval stages (Sugimoto, 2009a, 2009b). Also, males have wings, whereas most females cannot fly because of their wings have been reduced (Niitsu and Kobayashi, 2008; Roh et al., 2016).
In Korea, Roh et al. (2016) reviewed nine species including a new species, Psyche yeongwolensisByun and Roh, 2016 and a newly recorded species, Proutia maculatellaSaigusa and Sugimoto, 2014. Later, Roh and Byun (2016) recorded Ceratosticha leptodetaMeyrick, 1935 new for Korea. Recently, five more species were reported: Bacotia sakabeiSeino, 1981 (Roh and Byun, 2017a), Bruandella niphonica (Hori, 1926) and Proutia nigraSaigusa and Sugimoto, 2014 (Roh and Byun, 2017b), Dahlica (Dahlica) somae Roh and Byun, 2018 and Dahlica (Dahlica) ochrostigma Roh and Byun, 2018 (Roh et al., 2018). Consequently, 15 species in total are now known from Korea.
The genus Canephora, belonging to the subfamily Oiketicinae, was established by Hübner (1822) based on the type species Phalaena (Bombyx) unicolorHufnagel, 1766. Heylaerts (1900) described a new species, Chalia pungelerii from Japan. Later, Chalia pungelerii changed into Canephora pungelerii by Inoue (1981). Poda (1761) described a new species of Canephora, C. hirsuta from Austria. Thus, a total of two species of Canephora have been recorded worldwide to date (Sobczyk, 2011).
In this study, we report a newly recorded species of the genus Canephora, C. pungelerii from Korea. All available information is presented, including the collection locations, and illustration of female. DNA barcode is also provided for precise identification of each species.
Materials and Methods
The material examined in this study is preserved in the Systematic Entomology Laboratory, Hannam University (SEL/ HNU), Daejeon, Korea. Females were dissected and examined after mounting with euparal solution. Photographs were taken using a Pax cam digital camera (PAXcam Microscope Cameras Co., Chicago, IL, USA) attached to a Carl Zeiss Axio Imager A1 microscope (Carl Zeiss Ltd., Cambridge, MA, USA). Terminology and morphological characters of the females follows Sugimoto and Saigusa (2001).
Genomic DNA was extracted from the thorax part of an immersion specimen, preserved in 100% alcohol using a Genomic Cell/Tissue Spin Mini Kit (Mbiotech, Inc., Hanam, Korea), according to the manufacturer’s protocol. A specimen was sequenced for, the 658 bp fragment of the mitochondrial cytochrome c oxidase I (COI) gene, the DNA barcode, was amplified using the primer pair Lep F1 and Lep R1 (Hebert et al., 2004). PCR conditions for amplification followed the manufacturer’s protocol (Platinum Taq, Invitrogen, Carlsbad City, CA, USA). Amplicons were purified using the QIAquick® PCR purification kit (QIAGEN, Inc.) and directly sequenced at Genotech Corp. (Yuseong-gu, Daejeon, Korea). Contigs were assembled using CodonCode aligner version 2.0.6 (CodonCode Co., Centerville City, MA, USA). Successful sequences were submitted to GenBank.
Taxonomic accounts
Order Lepidoptera Linnaeus, 1758.
Family Psychidae Boisduval, 1829: 44.
Subfamily Oieketicinae Herrich-Schäffer, 1855: 48.
Genus Caenephora Hübner, 1822. 작은검정주머니나방속(신칭) Type species: Phalaena (Bombyx) unicolorHufnagel, 1766>
Canephorapugelerii (Butler, 1881) 작은날개검정주머니나방 (신칭)
Chalia pungelerii Heyaerts, 1900: 190. Type locality: Japan.
Eumeta pugeleri: Tutt, 1900: 434.
Eurycytarus andrewsi Wileman, 1911: 347.
Canephora pungeleri: Dierl, 1970: 170.
Canephora pungelerii: Inoue, 1981; 197-198.
Adult. Female (Fig. 1). Length 8.4-9.1 mm, vermiform, cylindrical, nearly same thickness from the metathorax to the 7th abdominal segment, 9 + 10th abdominal segments with short papillae anales. Coloration: Head with reddish brown and shiny vertex. Meso and metanotum red-brown. Membranous areas of abdomen yellowish brown. 7th abdominal segment pale yellow. Structure: Head (Fig. 1B) reduced in size, about 0.07 times as long as body length; apical part of vortex with few short hairs, mouth part slightly degenerated, antennae very short; well-developed with 2 segments, eye-spots blackish. Thorax (Fig. 1C and D) small, 0.16 times as long as body length, dorsal margin weakly curved, prothorax narrower than meso and metathorax, lateral part of metathorax with degenerated wings and few small setae, forewing narrow than hindwing. Legs almost degenerated with very short, present 2 segments (consist of 2 short segments, basal part with one femur and apical part with one tibia (Sugimoto and Saigusa, 2001)). Abdomen well developed, cylindrical and 5.14 times as long as body length, first abdominal segment well sclerotized, second to 6th abdominal segments almost membranous and second segment shorter than 3 to 6th segments, seventh abdominal segment nearly 1.4 times as long as the 6th segment and wide, dorsal part of 7th segment (corethrogyne or anal hair tuft) densely covered reddish brown hairs (but macerated condition), eighth abdominal segment very short, apophysis anterior parts invaginated from anterior margin of sclerite and very short, ostium bursa slightly thickness, 9th and 10th abdominal segments present papillae anales (ovipositor), papillae anales sclerotized and slightly short.
Male. Unknown.
Larval case (Fig. 2). It makes a relatively wide cylindrical case, which is slightly tapered to the lower part and covered with pieces of leafs arranged longitudinally.
Host plants (Fig. 2). It mainly feeds on Gramineae (Seino, 1975). In this study, Rhododendron indicum (L.) Sweet (Ericaceae) is reported for the first time from Korea as a host plant of C. pungelerii.
Material examined. 2♀, Jangneung, Yeongwol-gun, GW, 20. vi. 2014 (B.K. Byun and S.J. Roh), slide no. KNAEFSJ01; 2♀, Jeonmin-dong, Yuseong-gu, Daejeon, 20. v. 2015 (S.J. Roh), slide no. KNAEFSJ02; 1♀, Jeonmin-dong, Yuseong-gu, Daejeon, 1. vi. 2015 (S.J. Roh); 1♀, Jeonmin-dong, Yuseong-gu, Daejeon, 2. vi. 2015 (S.J. Roh), slide no. KNAEFSJ03-coll. SEL/HNU.
Distribution. Korea (new record), Japan.
DNA barcode. DNA barcode sequence was generated (accession no. MG563819). Multiple alignments using the BLAST tool in the NCBI database showed the following species as nearest neighbor: Canephorapugelerii (locality of reference data from Japan) 98%.
KSAE
