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ISSN : 1225-0171(Print)
ISSN : 2287-545X(Online)
Korean Journal of Applied Entomology Vol.49 No.4 pp.417-422

담배가루이(Bemisia tabaci) 병원성곰팡이 Lecanicillium lecanii Btab01 균주의 특성


Characterization of Lecanicillium lecanii Btab01 Isolated with Bioactivities to Tabacco Whitefly (Bemisia tabaci)

윤여준, 유용만, 이민호, 한은정, 홍성준, 안난희, 김용기, 지형진, 박종호


  Cultural characteristics Lecanicillium lecani Btab01 and its insecticidal activity against tobacco whitefly (Bemisia tabaci) were investigated. On potato dextrose agar, tryptic soy agar and SDA+Y media, mycelial growth of L. lecani Btab01 was best at 20~25℃ and suppressed above 28℃. Both solid culture and liquid culture of L. lecani Btab01 showed high insecticidal activity, 93.9 and 98.3% respectively, against nymph of tobacco whitefly, but there is no significant difference. When culture of L. lecani Btab01 was treated at the concentration of 10⁵, 10⁶, 10⁷ and 10⁸ cfu/ml, their insecticidal activity were 5.8%, 33.8%, 77.3% and 98.5% respectively, and LT50 values were 16.1 days, 7.3 days, 5.1 days and 3.5 days respectively. When nymphs were treated by the cultures of L. lecani Btab01 and maintained under saturated condition for zero hour, 24 hours and 168 hours, their control activities were 0%, 20.3% and 100% respectively. Spore germination of L. lecani Btab01 was increased about two times by adding edible oil. When L. lecani Btab01 was treated to control nymph with 0.1% edible oil, it showed high control activity (98.6%) compared to single treatment of L. lecani Btab01 (79.9%).