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ISSN : 1225-0171(Print)
ISSN : 2287-545X(Online)
Korean Journal of Applied Entomology Vol.48 No.4 pp.547-551
DOI :

팥나방(Matsumuraeses phaseoli)과 어리팥나방(M. falcana)의 판별 분자마커

초록

A Molecular Marker Discriminating the Soybean Podworm, Matsumuraeses phaseoli and the Podborer, M. falcana (Lepidoptera: Tortricidae)

허혜정, 손예림, 서보윤, 정진교, 김용균

Abstract

  Two closely related species, the soybean podworm, Matsumuraeses phaseoli, and the podborer, M. falcana, gives differential economic damages on crops. It is difficult to discriminate these potential sympatric species by morphological characters. The goal of this study was to develop a discriminating molecular marker based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A partial genomic fragment (500 bp) of mitochondrial cytochrome oxidaseⅠ (mtCOI) was sequenced in both species, in which restriction site by Rsa I was selected as a dichotomous marker. PCR-RFLP in the mtCOI region clearly discriminated both species.
Matsumuraeses phaseoli, Matsumuraeses falcana, PCR, RFLP, Molecular marker, 팥나방, 어리팥나방, 분자지표

Reference

Vol. 40 No. 4 (2022.12)

Journal Abbreviation Korean J. Appl. Entomol.
Frequency Quarterly
Doi Prefix 10.5656/KSAE
Year of Launching 1962
Publisher Korean Society of Applied Entomology
Indexed/Tracked/Covered By