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ISSN : 1225-0171(Print)
ISSN : 2287-545X(Online)
ISSN : 2287-545X(Online)
프루텔고치벌 브라코바이러스(Cotesia plutellae Bracovirus) 유래 IKB 유전자구조와 피기생 배추좀나방(Plutella xylostella) 체내 발현 패턴
초록
Gene Structure of Cotesia plutellae Bracovirus (CpBV)-IKB and Its Expression Pattern in the Parasitized Diamondback Moth, Plutella xylostella
Abstract
Inhibitor KB (IKB)-like gene has been found ill the genome of Cotesia plutellae bracovirus (CpBV), which is the obligatory symbiont of an endoparsitoid wasp, C. plutellae. The open reading frame of CpBV-IKB was 417 bp and encoded 138 amino acids. Four ankyrin repeat domains were found in CpBV-IKB, which shared high homology with other known polydnavirus IKBs. Considering a presumptive cellular IKB based on Drosophila Cactus, CpBV-IKB exhibited a truncated structure with deletion of signal-receiving domains, which suggested its irreversible inhibitory role in NFKB signal transduction pathway of the parasitized host in response to the wasp parasitization. CpBV-IKB was expressed only in the parasitized diamondback moth, Plutella xylostella. Its expression was estimated by quantitative RT-PCR during parasitization period, showing a constitutive expression pattern from the first day of parasitization. An indirect functional analysis of CpBV-IKB was conducted and suggested a hypothesis of host antivirus inhibition.
Antivirus, Cotesia plutellae, CpBV, IKB, NFKB, Plutella xylostella, Polydnavirus, 배추좀나방, 폴리드나바이러스, 프루텔고치벌, 항바이러스
Reference
Vol. 40 No. 4 (2022.12)
Frequency Quarterly
Doi Prefix 10.5656/KSAE
Year of Launching 1962
Publisher Korean Society of Applied Entomology
Online Submission
submission.entomology2.or.kr
KSAE
The Korean Society of Applied Entomology
