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ISSN : 1225-0171(Print)
ISSN : 2287-545X(Online)
ISSN : 2287-545X(Online)
Korean Journal of Applied Entomology Vol.65 No.3 pp.215-231
DOI : https://doi.org/10.5656/KSAE.2026.05.0.012
DOI : https://doi.org/10.5656/KSAE.2026.05.0.012
Transcriptomic Analysis of Gene Expression Changes in Thrips nigropilosus Uzel under Low-Temperature Exposure
Abstract
Thrips nigropilosus Uzel is a polyphagous thrips species causing economic damage to medicinal and horticultural crops, yet its molecular responses to low temperature remain unclear. Here, we analyzed the transcriptome of adults exposed to 4°C for 24 h (LOW) compared with a control at 25°C (CON). Illumina NovaSeq sequencing generated 7.38 × 108 paired-end reads, and de novo assembly produced 124,562 unigenes (N50 = 948 bp; average length = 611.4 bp). Functional annotation showed that 37.2% of unigenes matched at least one public database, with Gene Ontology terms mainly related to metabolic process and binding. Differential expression analysis identified 1,881 DEGs (860 up- and 1,021 down-regulated in LOW), indicating overall transcriptional suppression under cold stress. Cold-related genes, including acyl-CoA desaturase, cuticle protein, cytochrome P450, glutathione S-transferase, trehalose transporter, juvenile hormone esterase, and lipase, were significantly regulated, whereas vitellogenin was strongly down-regulated, suggesting a shift from reproduction to survival. RT-qPCR confirmed expression patterns consistent with RNA-seq results. This study provides the first transcriptomic resource characterizing the acute cold exposure response in T. nigropilosus and supports the development of improved thrips management strategies.
초록
Vol. 40 No. 4 (2022.12)
Frequency Quarterly
Doi Prefix 10.5656/KSAE
Year of Launching 1962
Publisher Korean Society of Applied Entomology
Online Submission
submission.entomology2.or.kr
KSAE
The Korean Society of Applied Entomology
